thr77 for Dummies
thr77 for Dummies
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and so may lead to tumorigenesis. This protein was found to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important function in marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two more splice variants ended up claimed but detailed nucleotide sequence info just isn't but readily available.
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide usually has the epitope identified from the antibody.
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This information outlines even further doable optimization of mobile and website immunoassay parameters to guarantee the very best final results are...
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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S phase boundary and is particularly degraded as cells development via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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